Abstract
A column chromatographic method for the determination of demethylchlortetra-cycline (DMCTC), tetracycline (TC), and chlortetracycline (CTC) was developed. The chromatogram involved the separation of these tetracyclines from their related compounds on a column of acid-washed diatomaceous earth treated with a buffer consisting of 0.1 M ethylenediaminetetraacetic acid disodium salt (EDTA) at pH 6.0 and pH 7.0, glycerin, and polyethylene glycol 400 (PEG 400), followe dby a modified spectral determination of column eluates. Although only DMCTC, TC, CTC, and their related compounds were studied in the work reported here, it appears reasonable to assume that the technique described could be extended to the assay of other tetracyclines. A column chromatographic method for the determination of demethylchlortetra-cycline (DMCTC), tetracycline (TC), and chlortetracycline (CTC) was developed. The chromatogram involved the separation of these tetracyclines from their related compounds on a column of acid-washed diatomaceous earth treated with a buffer consisting of 0.1 M ethylenediaminetetraacetic acid disodium salt (EDTA) at pH 6.0 and pH 7.0, glycerin, and polyethylene glycol 400 (PEG 400), followe dby a modified spectral determination of column eluates. Although only DMCTC, TC, CTC, and their related compounds were studied in the work reported here, it appears reasonable to assume that the technique described could be extended to the assay of other tetracyclines.
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