Abstract
BackgroundTetracarpidium conophorum is one of the numerous folklore medicinal plants for managing diabetes but the mode of action and bioactive compounds responsible for the antihyperglycemic property are missing in literatures. This study aimed at investigating the possible modes of its antihyperglycemic action using both in-vitro and ex-vivo methods. Powdered Tetracarpidium conophorum seed (TECOSE) was extracted with methanol using standard extraction procedure. Gas chromatography- Mass spectrometry (GCMS) analysis of the extract, and its effects on tissue glucose uptake, α-amylase, α-glucosidase and glucokinase enzymes were assessed using standard laboratory procedures.ResultsSeven heterocyclic compounds were identified by GCMS of which one is structurally related to sulphonylurea. TECOSE strongly inhibited α-glucosidase (IC50 = 1.90 mg/ml) but partially inhibited α-amylase (IC50 = 7.20 mg/ml) activities. Also, glucokinase activity and tissue glucose uptakes were significantly (p < 0.05) increased by TECOSE.ConclusionsThe results obtained deduced that antihyperglycemic action of TECOSE could be due to modulation of postprandial hyperglycaemia through inhibition of intestinal α-glucosidase, increasing glucokinase activity, improving peripheral glucose uptake by mimicking sulfonylurea action.
Highlights
Tetracarpidium conophorum is one of the numerous folklore medicinal plants for managing diabetes but the mode of action and bioactive compounds responsible for the antihyperglycemic property are missing in literatures
This study was aimed at identifying the bioactive compounds present in T. conophorum seed and assessing its biochemical effects on tissue glucose uptakes, α-amylase, α-glucosidase and glucokinase activities with a view to investigating its possible mode of action
Gas chromatography mass spectrometry (GCMS) analysis of Tetracarpidium conophorum seed extract Seven aromatic compounds were identified by Gas chromatography- Mass spectrometry (GCMS) analysis of T. conophorum seed extract (Table 1 and Fig. 1)
Summary
Tetracarpidium conophorum is one of the numerous folklore medicinal plants for managing diabetes but the mode of action and bioactive compounds responsible for the antihyperglycemic property are missing in literatures. This study aimed at investigating the possible modes of its antihyperglycemic action using both in-vitro and ex-vivo methods. Gas chromatography- Mass spectrometry (GCMS) analysis of the extract, and its effects on tissue glucose uptake, α-amylase, α-glucosidase and glucokinase enzymes were assessed using standard laboratory procedures. In Africa, many patients rely on traditional medicine because of the high cost of the synthetic drugs. This study was aimed at identifying the bioactive compounds present in T. conophorum seed and assessing its biochemical effects on tissue glucose uptakes, α-amylase, α-glucosidase and glucokinase activities with a view to investigating its possible mode of action
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