Abstract
To understand the significance of herbal drugs and the mechanism of their preparations, the knowledge of the bioactive chemical constituents of plants is required. The knowledge of the chemical constituents of plants would be valuable in discovering the actual potential/value of folkloric remedies. Gas chromatography and Mass spectroscopy (GC-MS) is considered as the most promising technique to identify or find out the exact chemical constituents in different plant extracts possessing medicinal activity. The current study was designed to find out the bio-active constituents present in methanol leaf extract of Achyranthes aspera by using Gas chromatography and Mass spectroscopy. GC-MS chromatograms of leaf extracts revealed the presence of numerous phyto-chemicals by showing different peaks of different heights. 12 major peaks were seen in methanol leaf extract of this plant by using this technique. The major chemical compounds/constituents were Phytol (21.99%), 9,12-Octadecadienoicacid (Z,Z) (13.74%) and 9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z) (13.35%). The occurrence of these active compounds in A. aspera may be associated directly or indirectly with its therapeutic potential.
 Keywords: Achyranthes aspera, methanol leaf extract, phytochemicals, Gas chromatography and Mass spectroscopy (GC-MS)
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