Test-System For Observing Heat Shock Protein 70kDa (Hsp70) Effect On Reactive Oxygen Species Production By Immune Cells.

Abstract

Event Abstract Back to Event Test-System For Observing Heat Shock Protein 70kDa (Hsp70) Effect On Reactive Oxygen Species Production By Immune Cells. NATALIA TROYANOVA1*, Ruslan Mirzoev1 and Aleksander Sapozhnikov1 1 Shemyakin–Ovchinnikov Institute of bioorganic chemistry RAS, Russia One of the most important aspects of the ROS biological activity is associated with the damaging effect on pathogens. But elevated ROS level can also lead to tissue damage. However, not all the mechanisms of ROS production regulation in cells are well investigated. We speculate that heat shock protein 70kDa (Hsp70) extracellular form may be one of the endogenous suppressors of the excessive ROS production by phagocytes. The aim was to determine antioxidant potential of Hsp70. Initially we have shown that 10 min heat stress treatment of the murine bone marrow cells with 60 min recovery at 37oC, prior to phorbol-12-myristate-13-acetate (PMA) activation, significantly reduced the intensity of the following ROS production compared with the control. Already after 5 min incubation in presence of Hsp70 we observed significant reduction of PMA-induced oxidative burst amplitude. The minimal concentration of Hsp70 required for the decrease of ROS production was strongly dependent from the incubation time and prolonged incubation decreased the effective Hsp70 concentration to 0.1 µg/ml. Strong dose-dependent effect of HSP70 was observed in the case of prolonged incubation. Besides effects on oxidative burst, Hsp70 reduces the level of spontaneous ROS production by the cells, which could be important in the process of aging regulation. In addition to the murine bone marrow model, the effect of HSP70 on oxidative burst was also demonstrated on human monocytes cell line THP-1. So, the antioxidant dose- and time-dependent effect of exogenously supplied Hsp70 on spontaneous and induced ROS production was shown using different cell models. Keywords: ROS, oxidative burst, Hsp70, Antioxidants, innate immunity Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: TROYANOVA N, Mirzoev R and Sapozhnikov A (2013). Test-System For Observing Heat Shock Protein 70kDa (Hsp70) Effect On Reactive Oxygen Species Production By Immune Cells.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00119 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. NATALIA TROYANOVA, Shemyakin–Ovchinnikov Institute of bioorganic chemistry RAS, Moscow, Russia, natywa@list.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers NATALIA TROYANOVA Ruslan Mirzoev Aleksander Sapozhnikov Google NATALIA TROYANOVA Ruslan Mirzoev Aleksander Sapozhnikov Google Scholar NATALIA TROYANOVA Ruslan Mirzoev Aleksander Sapozhnikov PubMed NATALIA TROYANOVA Ruslan Mirzoev Aleksander Sapozhnikov Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.