Abstract
Event Abstract Back to Event Expression of the galanin system in immune cells: hypothetical key players of the immune response. Rodolfo Bianchini1*, Andreas Koller1, Andreas Lang1, Silke Wiesmayr2, Bernhard Brodowicz1, Edith Mueller1 and Barbara Kofler1 1 Paracelsus Medical University, Department of Pediatrics, Austria 2 Paracelsus Medical University, Department of Pediatrics, Austria Neuropeptides participate in innate immunity and tolerance. Galanin is a neuropeptide with a widespread distribution in the central and peripheral nervous systems. Several in vivo studies indicate that galanin plays a role in inflammation. The aim of the present study was to identify immune cells expressing components of the galanin system including, not only the peptide but also its three receptors (GalR1-3). Quantitative RT-PCR analysis was performed on mRNA isolated from different types of human primary immune cells as well as human immune cell lines. We observed the expression of galanin in NK cells, skin mast cells, monocyte and macrophages. Accordingly, galanin expression was detected in the human mast cell line LDA2 and human monocyte cell line THP1. The analysis of GalR1, GalR2 and GalR3 revealed variable expression, which did not correlate with each other. GalR1 was only expressed by skin mast cells and THP1. GalR2 was detected in polymorphonuclear cells (PMNs), monocytes, macrophages, skin mast cells, LDA2 and THP1. GalR3mRNA expression was observed in monocytes, LDA2 and THP1. Moreover, flow cytometric analyses (FACS) revealed expression of GalR3 on T cells, monocytes, B cells and a minority of PMNs. Our results show the presence of galanin and GalRs in several types of immune cells. Experiments on different types of immune cells are under way to elucidate the functional role of galanin on immune cells expressing GalRs. Funding: Austrian Research Promotion Agency (FFG: 822782/THERAPEP) Keywords: Neuropeptides, immune cell expression, Galanin, galanin receptors, RT-PCR Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Bianchini R, Koller A, Lang A, Wiesmayr S, Brodowicz B, Mueller E and Kofler B (2013). Expression of the galanin system in immune cells: hypothetical key players of the immune response.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00807 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Rodolfo Bianchini, Paracelsus Medical University, Department of Pediatrics, Salzburg, Salzburg, A-5020, Austria, r.bianchini@salk.at Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rodolfo Bianchini Andreas Koller Andreas Lang Silke Wiesmayr Bernhard Brodowicz Edith Mueller Barbara Kofler Google Rodolfo Bianchini Andreas Koller Andreas Lang Silke Wiesmayr Bernhard Brodowicz Edith Mueller Barbara Kofler Google Scholar Rodolfo Bianchini Andreas Koller Andreas Lang Silke Wiesmayr Bernhard Brodowicz Edith Mueller Barbara Kofler PubMed Rodolfo Bianchini Andreas Koller Andreas Lang Silke Wiesmayr Bernhard Brodowicz Edith Mueller Barbara Kofler Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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