Testing of Volatile Oils Activity Extracted from Different Medicinal Plants Against Some Fungi Isolated from Different Sources

Hadeel A Al-Ameri Assist Prof

doi:10.33899/edusj.2021.168652

Abstract

Volatile oil extracted from Salvia officinalis showed significantly more effective e against growth of T. rubrum isolated from ulceration otitis externa with inhibition significant percentage 96.42% in concentration 1/25 then the concentration 1/50 with inhibition percentage 95.83%, two concentrations 1/75 and 1/100 showed low effects which inhibited fungal growth in percentage 89.28% and 81.54% respectively, While the volatile oil extracted from Zingiber officinale showed inhibited effect in percentage 92.26% in concentration 1/25 and 82.73% in concentration 1/50; The inhibition percentage was 79.76% and 77.97% in concentration 1/75 and 1/100 respectively. Volatile oil extracted from leaves of Apium graveolens was significantly more effective in concentration 1/25 then the volatile oil extracted from Zingiber officinale in the same concentration with a significant inhibition percentage 85.11% so volatile oil of leaves of Mentha piperita in concentration 1/25 with a significant inhibition percentage 81.54% and the concentration 1/100 from volatile oil of leaves of Mentha piperita Lower of all concentration inhibition of fungal growth above with inhibition percentage 70.23%. Volatile oils extracted from Salvia officinalis was more significant effect against growth of yeast C. albicans isolated from ulceration otitis externa so the inhibition percentage was 85.11% followed with volatile oil of leaves of Apium graveolens with percentage 80.35% in concentration 1/50 then volatile oil of leaves of Mentha piperita with inhibition percentage 79.16% in the concentration 1/25 while inhibition percentage of volatile oil extracted from Salvia officinalis ranged between 79.76% and 69.64%, While volatile oil of leaves of Apium graveolens inhibition percentage between 78.57% and 70.23% respectively in concentrations 1/25, 1/100; Volatile oil of Zingiber officinale was lower effect which inhibited fungal growth with percentage 78.57% and 61.30% in higher and lower concentrations respectively, Volatile oil extracted from Salvia officinalis showed significantly more effective e against growth of T. rubrum isolated from ulceration otitis externa with inhibition significant percentage 96.42% in concentration 1/25 then the concentration 1/50 with inhibition percentage 95.83%, two concentrations 1/75 and 1/100 showed low effects which inhibited fungal growth in percentage 89.28% and 81.54% respectively, While the volatile oil extracted from Zingiber officinale showed inhibited effect in percentage 92.26% in concentration 1/25 and 82.73% in concentration 1/50; The inhibition percentage was 79.76% and 77.97% in concentration 1/75 and 1/100 respectively. Volatile oil extracted from leaves of Apium graveolens was significantly more effective in concentration 1/25 then the volatile oil extracted from Zingiber officinale in the same concentration with a significant inhibition percentage 85.11% so volatile oil of leaves of Mentha piperita in concentration 1/25 with a significant inhibition percentage 81.54% and the concentration 1/100 from volatile oil of leaves of Mentha piperita Lower of all concentration inhibition of fungal growth above with inhibition percentage 70.23%. Volatile oils extracted from Salvia officinalis was more significant effect against growth of yeast C. albicans isolated from ulceration otitis externa so the inhibition percentage was 85.11% followed with volatile oil of leaves of Apium graveolens with percentage 80.35% in concentration 1/50 then volatile oil of leaves of Mentha piperita with inhibition percentage 79.16% in the concentration 1/25 while inhibition percentage of volatile oil extracted from Salvia officinalis ranged between 79.76% and 69.64%, While volatile oil of leaves of Apium graveolens inhibition percentage between 78.57% and 70.23% respectively in concentrations 1/25, 1/100; Volatile oil of Zingiber officinale was lower effect which inhibited fungal growth with percentage 78.57% and 61.30% in higher and lower concentrations respectively, While different volatile oils extracts shows a different inhibition effects against T. Mantagrophytes isolated from skin scraping, So volatile oil extracted from leaves of Mentha piperita showed more effective in concentration 1/25 with inhibition percentage 97.16% then volatile oil extracted from Salvia officinalis with percentage 77.38% in same concentration, There were no significant difference in concentration 1/25 and 1/50 of volatile oil extracted from Zingiber officinale with inhibition percentage 73.80% for both concentrations and volatile oil of leaves of Apium graveolens was less effective which inhibited T. Mantagrophytes in inhibition percentage 69.04% and 64.88% at the concentrations of 1/25 and 1/50 respectively. There was no significant difference between concentrations 1/75 and 1/100 with a percentage of 59.52% for both of them.

Highlights

Skin infections are a high percentage of skin diseases in humans, especially in warm areas where the environment is suitable for growth of fungi as well as the availability of moisture, heat and keratin materials [1], Skin fungi infect the surface layer of the body so it is Keratinophilic tissue [2] Which includes hair, skin and nails as keratin is the main source of nutrition [3], Filamentous dermatophytes produces many enzymes such as pyrotechnics, keratinase, elastase, lipase, phospholipids, amylase and DNA ase [4], and carbon and nitrogen [5]
Four medical plants were selected to extract the volatile oil from their parts and study their affectivity, Which are the leaves of Apium graveolens, Salvia officinalis and Zingiber officinale fruits displayed in local markets of Mosul city, While Mentha piperita leaves was obtained from the Gardens of Mosul university, the plant samples were classified in the herbivores of college of Science/Biology department /University of Mosul, Volatile oil attended from the parts of plants was studied by steam distillation
Source of isolates and method of sampling Trichophyton mentagrophytes and T. rubrum isolated from Scraps for skin by sterilizing the affected area superficially in ethanol 70% The edges of the infection were scraped using a sterile scalpel and the peels were taken and cultured on media Sabouraud's Glucose Agar (SGA) For three weeks, the fungi identification according to approved taxonomic keys

Summary

Introduction

Skin infections are a high percentage of skin diseases in humans, especially in warm areas where the environment is suitable for growth of fungi as well as the availability of moisture, heat and keratin materials [1], Skin fungi infect the surface layer of the body so it is Keratinophilic tissue [2] Which includes hair, skin and nails as keratin is the main source of nutrition [3], Filamentous dermatophytes produces many enzymes such as pyrotechnics, keratinase, elastase, lipase, phospholipids, amylase and DNA ase [4], and carbon and nitrogen [5]. Four medical plants were selected to extract the volatile oil from their parts and study their affectivity, Which are the leaves of Apium graveolens, Salvia officinalis and Zingiber officinale fruits displayed in local markets of Mosul city, While Mentha piperita leaves was obtained from the Gardens of Mosul university, the plant samples were classified in the herbivores of college of Science/Biology department /University of Mosul, Volatile oil attended from the parts of plants was studied by steam distillation.

Results

Conclusion