Temporal Changes in the Faecal Microbiota of Beef Cattle on Feedlot Placement.

Abstract

Simple SummaryMicroorganisms inhabiting the gut influence the health and productivity of livestock. Studies in ruminants including beef cattle show that ruminal microorganisms can help convert poor-quality or indigestible feed into nutrients, thereby influencing economically important traits like feed conversion efficiency and meat quality (e.g., marbling), and also interact with receptors on intestinal epithelial cells to regulate a variety of physiological processes including immune responses. Studies have also shown that the proportions of different groups of microorganisms in the gut are influenced by exposure to stress. In beef cattle, given that most cattle are finished on feedlots, induction into feedlots can expose cattle to stress due to transportation, change in diet and change in the handling and management of animals. Therefore, the primary objective of this study was to characterise how gut microorganisms change over time when cattle are inducted into feedlots. The results indicate that there are significant changes in the profiles of gut microorganisms in cattle that are visible soon after feedlot placement. These changes primarily include a reduced diversity of microorganisms within the gut of individual cattle and increased differences in the types of microorganisms that inhabit the gut of different cattle.The microbial communities that inhabit the intestinal tract play an important role in modulating health and productivity. Environmental stressors can impact microbial communities, which can significantly influence host physiology. Cattle are subjected to several environmental stressors when placed on feedlots, such as transportation stress, exposure to feedlot environments and change in diet and management. Exposure to these stressors could influence host gut microbiota, which in turn, could potentially influence host health and performance. The aim of the current study was to characterise the temporal changes that occur in intestinal microbiota as a consequence of feedlot placement by profiling 16s rRNA sequences in rectal faecal samples. When faecal microbiome profiles were compared in terms of relative abundances and alpha diversity metrics, the results showed significant, observable changes in profiles 2 days post-feedlot induction. Furthermore, beta-diversity analysis indicated that the phylogenetic similarity between samples significantly decreased on day 2 (PERMANOVA, p < 0.001). These trends were suggestive of a short-term reduction in microbial diversity coupled with decreased similarity between animals. These changes warrant further investigation and could provide opportunities for improved performance, health and even welfare of feedlot cattle in future.