TEMPO-Oxidized Cellulose Nanofiber-Alginate Hydrogel as a Bioink for Human Meniscus Tissue Engineering.

Xiaoyi Lan,Melanie Kunze,Yaman Boluk,Margaret J Vyhlidal,Alexander R A Szojka,Aillette Mulet-Sierra,Zhiyao Ma,Adetola B Adesida

doi:10.3389/fbioe.2021.766399

Xiaoyi Lan, Melanie Kunze + Show 6 more

Open Access

https://doi.org/10.3389/fbioe.2021.766399

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Abstract

Objective: The avascular inner regions of the knee menisci cannot self-heal. As a prospective treatment, functional replacements can be generated by cell-based 3D bioprinting with an appropriate cell source and biomaterial. To that end, human meniscus fibrochondrocytes (hMFC) from surgical castoffs of partial meniscectomies as well as cellulose nanofiber-alginate based hydrogels have emerged as a promising cell source and biomaterial combination. The objectives of the study were to first find the optimal formulations of TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl)-oxidized cellulose nanofiber/alginate (TCNF/ALG) precursors for bioprinting, and then to use them to investigate redifferentiation and synthesis of functional inner meniscus-like extracellular matrix (ECM) components by expanded hMFCs. Methods: The rheological properties including shear viscosity, thixotropic behavior recovery, and loss tangent of selected TCNF/ALG precursors were measured to find the optimum formulations for 3D bioprinting. hMFCs were mixed with TCNF/ALG precursors with suitable formulations and 3D bioprinted into cylindrical disc constructs and crosslinked with CaCl2 after printing. The bioprinted constructs then underwent 6 weeks of in vitro chondrogenesis in hypoxia prior to analysis with biomechanical, biochemical, molecular, and histological assays. hMFCs mixed with a collagen I gel were used as a control. Results: The TCNF/ALG and collagen-based constructs had similar compression moduli. The expression of COL2A1 was significantly higher in TCNF/ALG. The TCNF/ALG constructs showed more of an inner meniscus-like phenotype while the collagen I-based construct was consistent with a more outer meniscus-like phenotype. The expression of COL10A1 and MMP13 were lower in the TCNF/ALG constructs. In addition, the immunofluorescence of human type I and II collagens were evident in the TCNF/ALG, while the bovine type I collagen constructs lacked type II collagen deposition but did contain newly synthesized human type I collagen.

Highlights

The menisci are a pair of C-shaped fibrocartilages that withstand compressive and tensile forces (Fithian et al, 1990; Leslie et al, 2000; Liang et al, 2018)
Absorption band of carboxyl group (C O) stretching appeared around 1,650 cm−1, which is assigned to the formation of COO groups after the oxidation and release of nanofibers (El Bakkari et al, 2019)
In the steady-state flow sweep test (Figure 3A), all TEMPO-oxidized CNFs (TCNFs)/ALG precursors exhibited a shear-thinning behaviour, which may be attributed to the loss of chain entanglement as well as the alignment of polymeric chains/fibre under steady-state shear

Summary

Introduction

The menisci are a pair of C-shaped fibrocartilages that withstand compressive and tensile forces (Fithian et al, 1990; Leslie et al, 2000; Liang et al, 2018). They are essential for mechanical load distribution and transmission, lubrication, and stability of the knee joint (Makris et al, 2011; Liang et al, 2018). The cell population for the inner region of the meniscus are human meniscus fibrochondrocytes (hMFC), which are a mix of fibroblast and chondrocyte-like cells (King, 1936; Nakata et al, 2001; Szojka et al, 2021)

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