Temperature Dependencies of the Aggregation Properties of RBC in Dextran Solutions In Vitro

Petr B Ermolinskiy,Andrei E Lugovtsov,Anastasia I Maslyanitsina,Alexander V Priezzhev

doi:10.18287/jbpe20.06.020501

Abstract

The process of reversible spontaneous aggregation of red blood cells (RBC), as well as the process of their shear stress induced disaggregation, both affect hemorheology and blood microcirculation in the human body. The aim of this work is to estimate the influence of temperature on the aggregation properties of RBC suspended in PBS dextran solutions in vitro . Laser method based on diffuse light scattering was used to estimate the RBC aggregation properties. The results demonstrate a clear dependence of the critical shear stress aggregation parameter of RBC in PBS dextran solutions on temperature. These results would help to better understand the process of RBC aggregation .

Highlights

Processes of red blood cells (RBC) aggregation and disaggregation strongly influence the viscosity of blood in vessels and have a general impact human health [1, 2]
For dextran 150 kDa (Fig. 1b) the increase in temperature corresponds to shifting to the left, so that the maximum critical shear stress (CSS) is reached at lower concentration
The aggregation of RBC was studied by assessing the critical shear stress (CSS) that characterizes the hydrodynamic strength of the aggregates

Summary

Introduction

Processes of red blood cells (RBC) aggregation and disaggregation strongly influence the viscosity of blood in vessels and have a general impact human health [1, 2]. Aggregation properties of blood may be changed due many factors: alteration of blood plasma composition, change in blood temperature, injection of different synthetic macromolecules, RBC aging, pathological alterations, and others [2, 3]. The mechanisms of RBC aggregation, as well as the influence of these factors on it, are not fully understood so far. There exist two main hypotheses explaining the RBC aggregation process: the “Bridging” model and the “Depletion” model [2, 4, 5]. There exist some suggestions that the RBC aggregation process can be described as a combination of both models [6]. The development of the RBC aggregation models is vital for clearer predicting the changes of the microrheologic properties of blood due to the alterations of concentration of plasma proteins, temperature, etc. That is why the new knowledge about RBC aggregation process can be used for the development of future clinical applications

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Conclusion