Abstract
Red blood cell (RBC) aggregation highly influences hemorheology and blood microcirculation in the human body. The aggregation properties of RBCs can vary due to numerous factors, including RBC age. The aim of this work was to estimate in vitro the differences in the RBC aggregation properties of different RBC age populations in single-cell experiments using laser tweezers. RBCs from five healthy volunteers were separated into four subpopulations by Percoll density gradient centrifugation. Each subpopulation of the RBC was separately resuspended in autologous plasma or dextran 70 kDa (50 mg/mL). The aggregation force between the single cells was measured with holographic laser tweezers. The obtained data demonstrated an enhancement of RBC aggregation force in doublets with age: the older the cells, the higher the aggregation force. The obtained data revealed the differences between the aggregation and aggregability of RBC in dependence of the RBC in vivo age.
Highlights
Red blood cell (RBC) spontaneous aggregation and forced disaggregation processes strongly influence the microcirculation of blood and impact human health in general [1,2]
The number of the density enhancement of aggregation force (AF) with the increase in RBC age for RBCs in autologous plasma was found
It is important to mention that these data are from five healthy volunteers and for each volunteer of AF with the increase in RBC age for RBCs in autologous plasma was found (Figure 4a)
Summary
Red blood cell (RBC) spontaneous aggregation and forced disaggregation processes strongly influence the microcirculation of blood and impact human health in general [1,2]. In the “depletion” theory, the interaction between RBCs is described by osmotic forces that arise in the solution of macromolecules (e.g., proteins or synthetic macromolecules) surrounding the cells. In the “bridging” model, the interaction is described by the forces that arise due to the adsorption of macromolecules at the surface of RBC membrane and producing the bridges of these macromolecules between RBC membranes. To this day, there are strong arguments in favor of both models and there are some assumptions that both mechanisms influence the RBC aggregation [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
