Abstract
OBJECTIVE: This study was conducted to analyze the amounts of telomeric DNA and telomerase activity in early bovine embryos and, furthermore, to assess the usefulness as a marker to select a healthy embryo through the quantitative analysis of telomeric DNA depending on the embryonic grade.DESIGN: 29-46 embryos were used to analyze the amounts of telomeric DNA and 30-60 embryos to measure telomerase activity in early bovine embryos, 8 cell, morula and blastocyst.MATERIALS AND METHODS: The amounts of telomeric DNA in the early stages of bovine embroys were analyzed by Quantitative Fluorescence in situ Hybridization (Q-FISH) technique using a bovine telomeric DNA probe. Telomerase activity was analyzed by Telomeric Repeat Amplification Protocol (TRAP assay).Table 1The relative amount of telomeric DNA on in vitro fertilized bovine embryos.8 CellMorulaBlastocystNumber of analyzed embryos332946Number of analyzed blastomeres160544915Telomeric DNA in each cell(%)∗2.465±0.035c2.726±0.018b2.989±0.018aabcValues are means±SE. P<0.001. Open table in a new tab CONCLUSIONS: During early bovine embryogenesis, the relative amounts of telomeric DNA and the telomerase activity may represent a positive relationship. The telomerase activity seems to increase to maintain the amount of telomeric DNA. In addition, the increased amount of telomeric DNA and telomerase activity at blastocyst stage highly relates to the activity of embryonic development and differentiation. OBJECTIVE: This study was conducted to analyze the amounts of telomeric DNA and telomerase activity in early bovine embryos and, furthermore, to assess the usefulness as a marker to select a healthy embryo through the quantitative analysis of telomeric DNA depending on the embryonic grade. DESIGN: 29-46 embryos were used to analyze the amounts of telomeric DNA and 30-60 embryos to measure telomerase activity in early bovine embryos, 8 cell, morula and blastocyst. MATERIALS AND METHODS: The amounts of telomeric DNA in the early stages of bovine embroys were analyzed by Quantitative Fluorescence in situ Hybridization (Q-FISH) technique using a bovine telomeric DNA probe. Telomerase activity was analyzed by Telomeric Repeat Amplification Protocol (TRAP assay). abcValues are means±SE. P<0.001. CONCLUSIONS: During early bovine embryogenesis, the relative amounts of telomeric DNA and the telomerase activity may represent a positive relationship. The telomerase activity seems to increase to maintain the amount of telomeric DNA. In addition, the increased amount of telomeric DNA and telomerase activity at blastocyst stage highly relates to the activity of embryonic development and differentiation.
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