Abstract
Digital holographic microscopy (DHM) is a well-known technique utilized by researchers to precisely examine the 3D topography of microscopic objects. Until now, DHM systems have offered researchers a trade-off between either achieving telecentricity or maintaining a limited depth of focus, presenting a challenge for comprehensive 3D imaging. Here, we propose a very stable DHM system that utilizes a customized microscope objective and electrically tunable lens to enable rapid axial scanning within the extended depth of focus range, continuous field of view, and telecentricity at each image plane. An extended depth of focus up to 5.5 mm axially is obtained with consistent magnification and resolution with a minimal magnification error of 2.2 % over the entire axial range. Additionally, a thorough investigation into the temporal stability of the system was conducted, with multiple holographic recordings taken over a period of 7 s. The minimal deviation in object height, measuring just 0.31 nm (without the use of vibration isolation), underscores the good temporal stability inherent in this proposed DHM system.
Published Version
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