Teknik PCR Kualitatif untuk Deteksi Produk Rekayasa Genetika Jagung Event BT11 dan GA21

Abstract

<p>In some countries, including Indonesia, labelling of GMO products is mandatory for giving consumers the right to choose<br />between GMOs and conventional products. Therefore, development of methodology that can detect a specific genetically<br />modified (GM) crops and to verify the absence or presence of GM material in a product including raw materials (e.g. grains)<br />and/or their derivatives is needed. The objectives of this study were to find the most efficient screening methods to detect<br />whether or not a product is GM material and to develop a specific detection method to identify GM product BT11 and GA21. In<br />addition, present study was also aimed to obtain a duplex detection method for both GM products. Two GM-maize, including the<br />BT11 and GA21 lines of maize (Zea mays L.), and one plant, namely NK11 as the nontransgenic control, were used as plant<br />genetic materials in the event-specific detection of maize. The target gene from each sample was amplified in different reaction<br />(simplex) using both the event specific primer and the endogenous maize reference, Zein, as internal control. Furthermore, in<br />duplex PCR, two targets were simultaneously amplified in the same reaction. The results showed that detection method of the<br />GM product obtained from present study enabled us to screen the GM products and specifically the event of BT11 and GA21<br />using simplex and duplex methods. The duplex method is more efficient because it can detect two GM crops in one time<br />compared to simplex method that only can detect GM crop one by one.</p>