Techniques for the Isolation of Plant-Based Bioactive Compounds

Abstract

Phytochemicals are natural active ingredients that are occurring in plants. In the pursuit for novel biomolecules, the recovery of bioactive composites from plants, as well as their quantitative and qualitative evaluation, is critical. In the discovery and characterization of bioactive compounds, plant extraction and separation remain a critical concern. Analysis of bioactive compounds found in plant extracts using various approaches involving the use of chromatographic methods such as HPLC, TLC, OPLC, GC, PC, CC, and detection through FTIR, NMR, and MS is a frequent practice in the isolation of these bioactive compounds. The pure compound's structure and biological activity/bioactivity, such as antioxidant, antibacterial, or cytotoxicity, are then determined, along with their simplicity, specificity, and speed.