Abstract
Complex secondary metabolites which denature proteins during routine enzyme extraction are found in all tissues of most species of mangrove trees and prove a major impediment to isozyme studies of mangroves. An extraction procedure which neutralizes these substances is reported here together with methods for the starch gel electrophoresis of mangrove isozymes. Techniques described here allow assessment of variation within 14 enzyme systems, comprising 28 presumptive loci, in several species of Rhizophoraceae. Electrophoretic variation within five widely separated populations of the common Australian mangrove Rhizophora stylosa Griff. was conservative in terms of both proportion of polymorphic loci and numbers of alleles per locus, and showed little geographic variation.
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