Abstract
The staining of free hair samples is considered as one of the biggest problem by the Histologists. This technique has been developed for processing and routine staining of free hair samples without the skin. It is based on paraffin embedded blocks of hair samples. The blocks were cut in the rotary microtome at 6 µ thickness. After obtaining the sections of 6 µ thickness in rotary microtome, the sections were not required to be put in water bath as the hair samples were hard and did not get folded. Further, if water bath was used, this could result in the loss of the hair part from the section. The cuticle did not take any color in the routine staining as it was composed of enucleated or keratinized cells. The cortex had taken the pink colour of eosin which might be due to the composition of cortex with flattened cornified cells and numerous air spaces. The medulla showed a mixture of pink and blue color on staining with Hematoxylin and Eosin solutions.
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