Preparation of histology slides and photomicrographs: Indispensable techniques in anatomic education.

Abstract

The relevance of histology slides in microscopy cannot be overemphasized. However, a major challenge to many scientists in the field stems from paucity of published data to nearly lack of access to a comprehensive technical guide to prepare tissue samples as needed for practical demonstrations, reviews or diagnostic purposes. The current research project was carried out to produce histology glass slides and photomicrographs, as well as to document the step-by-step procedures for model by concerned practitioners and scientists. The standard procedures for paraffin method of histological preparation were employed. This involves sample fixation, dehydration, clearing, impregnation, embedding, section-cutting, staining and mounting. Harris hematoxylin and eosin were used for section staining. Tissue specimens were sourced from laboratory rats (Rattus norvegicus). Other materials used included reagents- formalin, alcohol, xylene, distyrene plasticizer xylene (DPX) as well as instruments/equipment- embedding mould, water bath, oven and rotary microtome. Photomicrographs were produced using digital camera attached to light microscope that is connected to a computer interface. 500 pieces set of slides comprising 20 different organ specimens was produced as samples, with each specimen mounted on 25 glass slides of either longitudinal or transverse sections. 40 digital images taken at low and high magnifications were also generated from the various specimens and subsequently stored on a disk. This project occupies a special place in practice due to the clarity about the techniques and the good quality of the slide series obtained which both contribute to knowledge.