Abstract
The translationally controlled tumor protein (TCTP) is an abundant, ubiquitous, and conserved protein which plays important roles in a number of biological processes. In the present study, the TCTP in shrimp Litopenaeus vannamei was analyzed. The TCTP of L.vannamei, a 168-amino-acid polypeptide, shares a high degree of similarity with TCTPs from other species, having two TCTP protein signatures at the 45–55 aa and 123–145 aa motif. The mRNA and protein levels from different tissues were detected with the highest in muscle and the lowest in heart among all examined tissues. In addition, temporal TCTP expression was significantly up-regulated at 16 h and 48 h following infection with white spot syndrome virus (WSSV). Lastly, silencing of TCTP with dsRNA led to a significant increase of WSSV loads. These results provide new insights into the importance of TCTP as an evolutionarily conserved molecule for shrimp innate immunity against virus infection.
Highlights
The translationally controlled tumor protein (TCTP) is initially described as a factor implicated in cell growth [1,2]
Especially by white spot syndrome virus (WSSV), are the greatest challenge to shrimp aquaculture
TCTP play an important role in the anti-stress program of many organisms, its roles in immune response is still remained limited, in invertebrates
Summary
The translationally controlled tumor protein (TCTP) is initially described as a factor implicated in cell growth [1,2]. It is a highly conserved 19-kDa protein, ubiquitously expressed in a wide range of eukaryotes, including yeast, plants, and animals [3,4,5,6]. In Venerupis philippinarum, the expression of TCTP was significantly decreased from 6 h to 12 h after infection, while it was up-regulated in that of 48 h [17]. All of these results suggest that TCTP may participate in a series of immune responses
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