Técnicas para el análisis de poblaciones de la molécula de fibrinógeno

Abstract

The purpose of this article is to analyze the integrity of fibrinogen purified by electrophoresis in a group of healthy people to obtain a reference standard considered normal to make comparisons possible with the results from people with coronary heart disease. An explanatory identification research was carried out with an experimental design based on the application of laboratory techniques for the analysis of various types of populations of the fibrinogen molecule in healthy people. The purification of the molecule was achieved by the method of Kazal (1963) with the subsequent performance of electrophoresis in acrylamide gel SDS-PAGE (Sodium dodecyl sulfate polyacrylamide gel electrophoresis by the technique of Laewmli (1970), obtaining the visualization of the main characteristic chains of the normal fibrinogen molecule (α, β, and γ). It is concluded that SDS-PAGE acrylamide gel electrophoresis allows the visualization of the main characteristic chains of the normal fibrinogen molecule (α, β, and) for later comparison with pathological patterns.