TBC1D3 family is a prognostic biomarker and correlates with immune infiltration in kidney renal clear cell carcinoma

Bei Wang,Dandan Chen,Haiying Hua

doi:10.1016/j.omto.2021.06.014

Abstract

The TBC1D3 family is overexpressed in many cancers, including kidney renal clear cell carcinoma (KIRC), which is associated with tumor-infiltrating lymphocytes. However, the expression and prognosis of TBC1D3 family and tumor-infiltrating lymphocytes in KIRC remain unknown. In the present study, we systematically explored and validated the expression and prognostic value of TBC1D3 family expression in KIRC using multiple public databases. In addition, the function of the TBC1D3 family members and the correlations between TBC1D3 family expression and KIRC immune infiltration levels were investigated. We found that TBC1D3 family members were rarely mutated (less than 5 frequencies). TBC1D3 family was overexpressed in KIRC; high expression of the TBC1D3 family members was correlated with poor prognosis. In addition, TBC1D3D may positively regulate proliferation, and overexpression of TBC1D3 promoted clear cell renal cell carcinoma proliferation in vitro. In terms of immune infiltrating levels, TBC1D3 family expression was positively associated with CD4+ T cells infiltrating levels. These findings suggest that the TBC1D3 family expression is correlated with prognosis and immune infiltrating levels. Therefore, the TBC1D3 family can be used as a biomarker for KIRC and a prognostic biomarker for determining the prognosis and immune infiltration levels in KIRC.

Highlights

Clear cell renal cell carcinoma, or kidney renal clear cell carcinoma (KIRC), is the most common malignant tumor of renal cancer, accounting for 75%–82% of primary malignancies in the kidney.[1]
The results showed that the TBC1D3 expression was respectively higher in KIRC, KIRP, LIHC, LUAD, LUSC, and THCA
KIRC has been shown to have the highest level of immune infiltration and T cell infiltration.[2]

Summary

Introduction

Clear cell renal cell carcinoma (ccRCC), or kidney renal clear cell carcinoma (KIRC), is the most common malignant tumor of renal cancer, accounting for 75%–82% of primary malignancies in the kidney.[1] In a variety of clinical and genomic studies, KIRC has been shown to be a highly immune-infiltrated tumor, and KIRC is one of the earliest malignancies to respond to immune therapy.[2]. TBC1D3 acts as a GTPase-activating protein for RAB5, and TBC1D3 is identified as a novel nucleocytoplasmic protein, which is regulated by the microtubule network.[6] Recently, TBC1D3 was found to promote breast cancer cell migration.[7] the function of the TBC1D3 family in KIRC remains unknown. We explore the expression, prognosis, and tumor infiltrating lymphocytes of the TBC1D3 family in KIRC through multiple databases

Methods

Results

Conclusion