TaYS1A, a Yellow Stripe-Like Transporter Gene, Is Required for Wheat Resistance to Puccinia striiformis f. sp. Tritici.

Md Ashraful Islam,Jia Guo,Xingxuan Bai,Jun Guo,Zhensheng Kang,Haochuan Zhu,Huan Peng,Shuxin Tian

doi:10.3390/genes11121452

Md Ashraful Islam, Jia Guo + Show 6 more

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https://doi.org/10.3390/genes11121452

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Journal: Genes	Publication Date: Dec 3, 2020
Citations: 8	License type: CC BY 4.0

Affiliation: North West Agriculture and Forestry University

Abstract

Yellow stripe-like (YSL) transporters are required for the transportation of metal-phytosiderophores and are structurally related to metal-nicotianamine complexes. Some studies also reported the involvement of YSL transporters in pathogen-induced defense. However, the molecular mechanisms of YSL genes involved in biotic stress responses are still not clear, especially in cereal crops. This study aimed to functionally characterize TaYS1A during the interaction of wheat and Puccinia striiformis f. sp. tritici (Pst), the causal agent of stripe rust disease. TaYS1A was localized in the cell membrane of wheat protoplasts and Nicotiana benthamiana cells. TaYS1A was significantly up-regulated in wheat leaves after being infected with the avirulent Pst isolate CYR23 and after treatment with salicylic acid (SA). Silencing of TaYS1A by the virus-induced gene silencing method enhanced the susceptibility of wheat to Pst accompanied by reducing the accumulation of SA and H2O2 and down-regulating the transcriptions of TaPR1 and TaPR2. In addition, TaYS1A was found to interact with TaNH2, a homolog of OsNH2, by yeast-two-hybrid assay, and silencing of TaYS1A diminished the expression of TaNH2. Our findings suggested the existence of positive regulation of TaYS1A in providing resistance against Pst by modulating SA-induced signaling and offered new insight into the biological role of YSL in wheat against pathogens.

Highlights

Puccinia striiformis f. sp. tritici (Pst), causing wheat stripe rust, is a major challenge to global wheat production
Plants produce a sequence of immune responses to defend against pathogen invasion, such as ion fluxes through the plasma membrane, increased intracellular Ca2+ concentration, accumulation of reactive oxygen species (ROS), biosynthesis of salicylic acid (SA), fortification of the cell-wall, a hypersensitive response (HR), the formation of localized cell wall appositions (CWAs or papillae), among others [1,2,3,4]
Our results suggest that the silencing of in wheat benefits significantly increased at 48 hpi, and the infection area significantly expanded at 120 the hpi growth development of Pst, andour increases wheat avirulent and virulent

Summary

Introduction

Pst, causing wheat stripe rust, is a major challenge to global wheat production. One of the most economical and productive strategies for stripe rust management is to develop resistant wheat cultivars. Dissection of the molecular interaction pathways between wheat and Pst would promote the appropriate use of resistance genes for varietal improvement. Plants produce a sequence of immune responses to defend against pathogen invasion, such as ion fluxes through the plasma membrane, increased intracellular Ca2+ concentration, accumulation of reactive oxygen species (ROS), biosynthesis of salicylic acid (SA), fortification of the cell-wall, a hypersensitive response (HR), the formation of localized cell wall appositions (CWAs or papillae), among others [1,2,3,4]. Beyond the establishment of CWAs, ROS are involved in several plant signaling regulations including local and systemic signaling essential for

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