Taxonomy and Phylogeny of Polyporus Group Melanopus (Polyporales, Basidiomycota) from China.

Jun-Liang Zhou,Bao-Kai Cui,Hong Chen,Lin Zhu,Tamás Papp

doi:10.1371/journal.pone.0159495

Jun-Liang Zhou, Bao-Kai Cui + Show 3 more

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https://doi.org/10.1371/journal.pone.0159495

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Journal: PloS one	Publication Date: Aug 3, 2016
Citations: 32	License type: CC BY 4.0

Affiliation: Beijing Forestry University, Institute of Microbiology

Abstract

Melanopus is a morphological group of Polyporus which contains species with a black cuticle on the stipe. In this article, taxonomic and phylogenetic studies on Melanopus group were carried out on the basis of morphological characters and phylogenetic evidence of DNA sequences of multiple loci including the internal transcribed spacer (ITS) regions, the large subunit nuclear ribosomal RNA gene (nLSU), the small subunit nuclear ribosomal RNA gene (nSSU), the small subunit mitochondrial rRNA gene sequences (mtSSU), the translation elongation factor 1-α gene (EF1-α), the largest subunit of RNA polymerase II (RPB1), the second largest subunit of RNA polymerase II (RPB2), and β-tubulin gene sequences (β-tubulin). The phylogenetic result confirmed that the previously so-called Melanopus group is not a monophyletic assemblage, and species in this group distribute into two distinct clades: the Picipes clade and the Squamosus clade. Four new species of Picipes are described, and nine new combinations are proposed. A key to species of Picipes is provided.

Highlights

Melanopus Pat. was established by Patouillard [1] as a specific genus which including stipitate polypores with black stipes
An 8-gene concatenated dataset resulted in an alignment with 7270 total characters was carried out
Picipes baishanzuensis was collected from subtropical area of China

Summary

Introduction

Melanopus Pat. was established by Patouillard [1] as a specific genus which including stipitate polypores with black stipes. It was accepted as a synonym of Polyporus P. Núñez and Ryvarden [3] treated genus Melanopus as an infrageneric group of Polyporus. They defined this group with following characters: basidiocarps coriaceous, tough when dry, context thin, stipe with a black cuticle, skeleto-binding hyphae mostly solid and narrow when mature, basidiospores medium size to large (6–12 × 2–4 μm). Eleven species of Polyporus were accepted as members of group Melanopus by Núñez and Ryvarden [3] Among these species, P. mikawai Lloyd was transferred into Neofavolus Sotome & T. Hatt. based on morphological and phylogenetic analyses [4]

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