Abstract
RPM1 is a CC-NBS-LRR protein that was first shown to be required for resistance to Pseudomonas syringae pv. maculicola in Arabidopsis thaliana. Our previous study showed that TaRPM1 gene in wheat was upregulated about six times following infection by Puccinia striiformis f. sp. tritici (Pst) under high temperature, compared with normal temperature. To study the function of TaRPM1 in wheat high-temperature seedling-plant (HTSP) resistance to Pst, the full length of TaRPM1 was cloned, with three copies each located on chromosomes 1A, 1B, and 1D. Transient expression of the TaRPM1-GFP fusion protein in Nicotiana benthamiana indicated that TaRPM1 localizes in the cytoplasm and nucleus. Profiling TaRPM1 expression indicated that TaRPM1 transcription was rapidly upregulated upon Pst inoculation under high temperature. In addition, TaRPM1 was induced by exogenous salicylic acid hormone application. Silencing TaRPM1 in wheat cultivar Xiaoyan 6 (XY 6) resulted in reduced HTSP resistance to Pst in terms of reduced number of necrotic cells and increased uredinial length, whereas no obvious phenotypic changes were observed in TaRPM1-silenced leaves under normal temperature. Related defense genes TaPR1 and TaPR2 were downregulated in TaRPM1-silenced plants under high temperature. We conclude that TaRPM1 is involved in HTSP resistance to Pst in XY 6.
Highlights
Wheat (Triticum aestivum) yield can be greatly reduced by stripe rust caused by obligate biotrophic pathogen Puccinia striiformis f. sp. tritici (Pst) (Wellings, 2011; Chen, 2014)
We identified and cloned a highly upregulated nucleotide-binding sites (NBS)-leucine-rich repeats (LRR) gene TaRPM1 from Xiaoyan 6 (XY 6) infected with Pst and subsequently exposed to high temperature for 24 h
The predicted amino acid sequence of this protein was identical to that of AetRPM1-like of Aegilops tauschii; this gene is named as TaRPM1 (GenBank accession number MN647923)
Summary
Wheat (Triticum aestivum) yield can be greatly reduced by stripe rust caused by obligate biotrophic pathogen Puccinia striiformis f. sp. tritici (Pst) (Wellings, 2011; Chen, 2014). The loss of resistance in wheat cultivars has been happening frequently due to rapid emergence of new virulent races in Pst (Chen et al, 2009; Hu et al, 2014). Wheat high-temperature (HT) resistance to Pst, a non-racespecific and durable resistance, can be classified into two types: high-temperature adult-plant (HTAP) and high-temperature seedling-plant (HTSP) resistance (Chen, 2013). HTAP resistance expresses or increases when plants are in the adult stage and weather becomes warm, whereas HTSP resistance expresses when wheat seedlings are temporarily exposed to 20°C for only 24 h at the initial stage of Pst incubation (Wang et al, 2017a; Wang et al, 2019). Xiaoyan 6 (XY 6) is a typical example of wheat cultivars with HTSP resistance to Pst
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