Abstract
Chemotherapeutic drugs, such as cyclophosphamide, cause severe immunosuppression and patients become susceptible to infections. Based on this, the immunomodulatory potential of tarin, a lectin from Colocasia esculenta, was evaluated in bone marrow cell cultures and in cyclophosphamide-immunosuppressed mice. Tarin promoted maintenance of hematopoietic progenitors and repopulation of Gr1 cells in vitro which was supported by in vivo results. In immunosuppressed mice, tarin increased bone marrow cell numbers and altered cell profile distribution by enhancing the frequency of Gr1+ progenitors, including Ly6-CintLy6-Glo, and anticipating their proliferation/differentiation in mature cells, especially Ly6-CloLy6-Ghi. Bone marrow cells harvested from tarin-treated immunosuppressed mice proliferated in response to GM-CSF or G-CSF in vitro and, the low numbers of bone marrow cells in the G0 phase, combined with a high number cells undergoing apoptosis confirmed that tarin promoted a faster and intense proliferation/differentiation, even in the presence of CY-induced toxicity. As a result, tarin minimized leukopenia in immunosuppressed mice promoting a faster recovery of peripheral leucocytes and protected erythroid bone marrow cells from CY-cytotoxicity in a dose-dependent manner. Data suggest that tarin could be considered a potential adjuvant to decrease leukopenia and possibly ameliorate anemia, if carefully evaluated in human cancer cell lineages and in clinical trials.
Highlights
Bone marrow (BM) cells were obtained on day 4 from distinct mice groups: CY–mice immunosuppressed with CY 300 mg/kg (Genuxal) (Baxter Hospitalar Ltda, MG, BRA); CY+Tarin—CY-immunosuppressed mice treated concomitantly with 200 μg tarin on day 0; Tarin—mice treated with 200 μg tarin on the same day or Control—mice inoculated with saline
To study the effects of tarin administration on the cell cycle and apoptosis, BM cells were obtained from mice on day 4 after as follows: CY–mice immunosuppressed with CY 300 mg/ kg; CY+Tarin—CY-immunosuppressed mice treated concomitantly with 200 μg tarin on day 0; Tarin -mice treated with 200 μg tarin on the same day or Control—mice inoculated with saline
Tarin exhibited protective and stimulatory effect on mice bone marrow cells in vitro To investigate the immunomodulatory potential of tarin, mice BM cells were cultured in the presence of tarin and the granulocytes was evaluated by cytospin and flow cytometry
Summary
Adult male C57BL/6 mice (8 to 12 weeks old) were provided by the Laboratory Animal Nucleus (NAL), located at the Biology Institute of the Universidade Federal Fluminense (UFF), Brazil. Research protocol was approved by the Animal Experimentation Ethics Committee (CEPA) at NAL-UFF, under number 670/2016. Colocasia esculenta (L.) Schott corms were manually chosen and purchased from a local market in Rio de Janeiro, Brazil. The crude taro extract (CTE) was obtained according to Roy, Banerjee, Majumder, & Das [14] and was stored at –20 ̊C until tarin purification steps. Tarin purification was performed according to the protocol described previously by Pereira et al [7], by affinity chromatography through a Cibacron Blue 3GA (Sigma-Aldrich Co, MO, USA) column. Protein concentrations of the tarin fractions were estimated by the Lowry method [15], using bovine serum albumin (BSA) (Sigma-Aldrich Co) at 1mg/mL for the standard curve
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
