Targeted identification of camellia oil and olive oil adulterated with sesame or rice oil based on characteristic substances by HPLC-UV

Abstract

This paper aims to establish a rapid and accurate method to identify the adulteration of camellia oil and olive oil based on characteristic substances. The results showed that the sesamin and sesamolin can be selected as the characteristic substances of sesame oil, and oryzanol is identified as the characteristic substances of rice oil. A high-performance liquid chromatography (HPLC) method with UV determination was developed to detect the characteristic substances, which can identify whether sesame oil and rice oil are mixed in camellia oil or olive oil. The pretreatment and chromatographic conditions were optimized. The linear equation, LOD, LOQ, recovery and precision were studied. The LODs of sesamin, sesamolin and oryzanol were determined to be 25 mg/kg, 50 mg/kg and 50 mg/kg, respectively. Similarly, the LOQs of sesamin, sesamolin and oryzanol were 50 mg/kg, 100 mg/kg and 100 mg/kg respectively. The simulated adulterated oils with different proportion were analyzed to validate the effectiveness of method. The adulteration limit of sesame oil is 2 %, and that of rice oil is 5 %. The method has the advantages of simple operation, good specificity and high accuracy. It can provide technical support for oil quality control and adulteration identification.