Abstract
Homology-dependent gene silencing (HDGS) is a resistance mechanism against homologous transgene introduction or virus infection in various organisms. In plants in particular, HDGS phenomena in homologous DNA or RNA sequences can cause gene silencing. In this study, GUS-silenced transgenic lines were developed by agroinfiltration with the i35S binary vector for inducing targeted de novo CaMV 35S promoter methylation. Agroinfiltration with i35S triggered inheritable de novo methylation of the CaMV 35S promoter and CAAT box region by HDGS. Moreover, targeted de novo CaMV 35S promoter methylation appeared to be controlled by the expression of SUVH9, which is involved in endogenous DNA methylation and hyper-methylation of the introduced promoter. In summary, this study demonstrated that targeted de novo CaMV 35S promoter methylation may be subjected to an HDGS mechanism that can downregulate gene expression and maintain the heritable stability of the methylated promoter.
Published Version
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