Abstract
The hematological parameters red blood cells (RBC) and total white blood cells (WBC) counts, hematocrit, hemoglobin concentration, and RBC indexes (median corpuscular volume and median corpuscular hemoglobin concentration) were determined and T CD5+ lymphocytes and CD4+ and CD8+ subpopulations of the umbilical cord blood (UCB) of dogs were quantified by the cytofluorimetric technique. Nine adult Beagles, from two do five-year old, were used as control. The umbilical cord blood (UCB) was collected from 20 neonate dogs. The method for the UCB collection was adequate to obtain sufficient quantity of blood for the accomplishment of the hematological analyses and lymphocyte quantification. Cytoscopic preparations of the UCB suggested high erythropoietic activity. There was no difference for the global leukocyte and lymphocyte counts between the groups. UCB T lymphocyte counts were lower than those obtained for adult dogs. The proportion of CD4:CD8 showed a great dominance of T CD4+ cells over T CD8+ lymphocytes in UCB.
Highlights
The umbilical cord blood (UCB) is a rich source of stem cells with high capacity of selfrenovation and proliferation (Dimitriou et al, 1998)
After collection, the umbilical cord blood and the peripheral blood were submitted to laboratory analyses, including red blood cells (RBC) and total white blood cells (WBC) counts, hematocrit (HCT), hemoglobin (Hgb) concentration, and the RBC indexes median corpuscular volume (MCV) and median corpuscular hemoglobin concentration (MCHC), using an automatic cell counter1
The global counting of red blood cells and the MCHC obtained for the UCB were inferior to those of the peripheral blood of adult dogs (P
Summary
The umbilical cord blood (UCB) is a rich source of stem cells with high capacity of selfrenovation and proliferation (Dimitriou et al, 1998). Researches have revealed that there is a lower quantity of T CD3, CD4 and CD8 lymphocytes in the human UCB, when compared to the adult peripheral blood, as well as a higher proportion of CD4:CD8 (Dimitriou et al, 1998; Motley et al, 1996).
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call