Abstract
Ca2+ release-activated Ca2+ (CRAC) channel-mediated Ca2+ entry regulates multiple T lymphocyte functions. T cells activated by crosslinking of T cell receptors display enhanced Ca2+ signaling compared with resting T cells; this can be caused by activation-induced upregulation of CRAC channel expression. However, studies of the expression of Orai and Stim family genes encoding CRAC channel structural elements and regulatory proteins, respectively, produced controversial results. Using quantitative RT-PCR assay we re-examined Orai and Stim gene expression relative to the stably expressed B2M and RPL13a reference genes in resting, activated, and Jurkat T cells.
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