Abstract
BackgroundSaliva (oral fluids) is an emerging biofluid poised for detection of clinical diseases. Although the rationale for oral diseases applications (e.g. oral cancer) is intuitive, the rationale and relationship between systemic diseases and saliva biomarkers are unclear.Methodology/Principal FindingsIn this study, we used mouse models of melanoma and non-small cell lung cancer and compared the transcriptome biomarker profiles of tumor-bearing mice to those of control mice. Microarray analysis showed that salivary transcriptomes were significantly altered in tumor-bearing mice vs. controls. Significant overlapping among transcriptomes of mouse tumors, serum, salivary glands and saliva suggests that salivary biomarkers have multiple origins. Furthermore, we identified that the expression of two groups of significantly altered transcription factors (TFs) Runx1, Mlxipl, Trim30 and Egr1, Tbx1, Nr1d1 in salivary gland tissue of melanoma-bearing mice can potentially be responsible for 82.6% of the up-regulated gene expression and 62.5% of the down-regulated gene expression, respectively, in the saliva of melanoma-bearing mice. We also showed that the ectopic production of nerve growth factor (NGF) in the melanoma tumor tissue as a tumor-released mediator can induce expression of the TF Egr-1 in the salivary gland.ConclusionsTaken together, our data support the conclusion that upon systemic disease development, significant changes can occur in the salivary biomarker profile. Although the origins of the disease-induced salivary biomarkers may be both systemic and local, stimulation of salivary gland by mediators released from remote tumors plays an important role in regulating the salivary surrogate biomarker profiles.
Highlights
Saliva harbors a wide spectrum of proteins/peptides, nucleic acids, electrolytes, and hormones that originate in multiple local and systemic sources
Taken together, our data support the conclusion that upon systemic disease development, significant changes can occur in the salivary biomarker profile
The origins of the disease-induced salivary biomarkers may be both systemic and local, stimulation of salivary gland by mediators released from remote tumors plays an important role in regulating the salivary surrogate biomarker profiles
Summary
Saliva harbors a wide spectrum of proteins/peptides, nucleic acids, electrolytes, and hormones that originate in multiple local and systemic sources. A number of findings in the past decade have prompted interest in the use of saliva as a source of biomarkers. The soluble fragment of c-erbB-2 was detectable in the saliva of breast cancer patients but not in healthy controls or patients bearing benign tumors [3]. In 2004 saliva-based HIV detection was approved by the US Food and Drug Administration (FDA). Saliva (oral fluids) is an emerging biofluid poised for detection of clinical diseases. The rationale for oral diseases applications (e.g. oral cancer) is intuitive, the rationale and relationship between systemic diseases and saliva biomarkers are unclear
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