Synthetic CsCEP3 Peptide Attenuates Salinity Stress via ROS and ABA Signaling in Cucumber Primary Root

Abstract

Salinity stress prominently limits cucumber growth and productivity. However, the mechanism underlying salinity response mediated by the C-TERMINALLY ENCODED PEPTIDE (CEP) peptide in cucumber primary root remains largely unclear. In this study, we show that salinity prominently inhibits cucumber primary root growth, and CsCEP gene expression is differentially induced by salinity. We further demonstrate that the exogenous application of synthetic CsCEP3 peptide partially suppresses salinity-triggered growth inhibition in cucumber primary root, although CsCEP3 peptide itself shows no obvious effect on cucumber primary root growth under normal conditions. Our transcriptomic and qRT-PCR data further reveal that CsCEP3 peptide may modulate gene expression related to abscisic acid (ABA) signaling pathway, reactive oxygen species (ROS) production, and slat-responsive transcription factors to attenuate the inhibitory effect of salinity on cucumber primary root growth. Taken together, our work provides a fundamental insight into CEP peptide-mediated cucumber salinity adaptation.