Synthesis properties and matrix binding of fe‐porphyrins containing isocyanate side‐chains

Abstract

AbstractFixation of the prosthetic groups of hemoproteids on synthetic macromolecular carriers is important from the viewpoint of biocatalytic investigations.Fe‐porphyrins which contained isocyanate sidechains have been prepared by two methods. This isocyanate reacted in pyridine as the solvent at room temperature with cross‐linked poly(2‐hydroxyethylmethacrylate) and with Sephadex LH 20, a hydroxypropyl group containing a carrier.The identity of the new Fe‐porphyrin derivatives and coupling products with synthetic carriers could be ascertained by elementary analysis and by absorption spectroscopy in the visible and infrared region.The properties of mesohemin IX are retained after covalent binding to the carriers. At near neutral pH, a spectrum typical of alkaline hematin (parahematin) occurred. At pH 1.6, the spectrum of acidic hematin appears. The ligand binding ability to CN− and imidazole has been retained. The reduction of the Fe(III) with sodium dithionite is possible. The coupling products show peroxidatic activity.