Abstract
Polio- (type 1, Brunhilde), ECHO- (type 19), and coxsackieviruses (types A7, A9 and B6) when grown in monkey kidney tissue culture (MKTC), LLCMK2 or WI-38 cells, yielded virus stocks containing virions and uncoated viral RNA. These viruses grown in a number of other cells (for example KB, HeLa, AV-3, HEp-2 or human amnion) contained no demonstrable uncoated RNA. Homogenates of the latter cells, however, inactivated infectious polio RNA, whereas homogenates from MKTCs, LLCMK2 and WI-38 cells did not. In virus stocks prepared in MKTC about 103 PFU/ml. of uncoated infectious RNA was found. Studies on the temporal appearance of uncoated polio RNA in MKTCs, HEp-2 and HeLa cells revealed extracellular emergence of uncoated RNA between the 8th and 15th hour after infection, reaching maximum yields after 24 hours. In the MKTC system uncoated RNA persisted, whereas in the HEp-2-system, it was slowly inactivated and completely destroyed within 72 hours (3 days) after infection; in HeLa cells it was inactivated at a much faster rate and never reached high infectivity titers.
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