Abstract
Poliovirus RNA has been chromatographed previously. Kubinsl~y and Koch (1) using methylated serum albumin on celite as column packing separated polio RNA from cellular RNA. Lamb and Dubes (2) using a column of hydromagnesite separated poliovirus RNA from intact virions, cellular RNA, DNA, and glycogen. Both methods are based on ion exchange; a gradient with increasing ionic strength is used to elute the substances adsorbed to the column. Gel filtration does not require an eluent gradient and the eluent can be chosen according to the necessities dictated by the further use of the fractions. In the ease of virus and RNA ffactionation phosphate buffered saline (PBS) was used as eluent because the infectivity residing in the various fractions could be determined on monkey kidney tissue cultures (MKTC's) without further adjusting the fractions to physiological conditions. The Sephadex gels separate substances primarily on the basis of differences of their molecular weight (MW) but also on differences in their struetual configuration or chemical compo-
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