Abstract
AbstractBeside the predominately found 8‐(arylamino)‐2′‐dG, 8‐(acetylarylamino) damages within DNA‐strands may also play an important role in the induction of chemical carcinogenesis. A synthesis pathway leading to these 8‐(acetylarylamino)‐dG adducts using different aromatic amines has been optimized. The 8‐modified dGs were converted into the corresponding phosphoramidites and site‐specifically incorporated into different oligonucleotides leading to DNA strands. Lesion‐bearing hybrids of these damaged DNA‐strands with complementary oligonucleotides were used to study their melting properties and their circular dichroism spectra. It was shown that no EcoRI restriction took place with the damage inside the cleavage site. Finally, three different DNA polymerases were used for primer extension studies.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call