Synthesis of Silver Nano particles by Green method Using Vernonia anthelmintica and its Antibacterial activity.

Abstract

In the modern years, there is an enlarged use of silver nanoparticles (SNPs) in industrial and in the field of medical practice significantly, still, their high risk and lethal outcome have not been studied considerably. The aim behind this research work was to incorporate silver nanoparticles (AgNPs) using Vernonia anthelmintic seeds extract and to assess its antibacterial activity. AgNPs were properly synthesized by the green method and the evolution of AgNPs was firmed by optical color change and UV (ultraviolet) spectroscopy. Synthesized AgNPs were decontaminated and characterized by using Silver Content Estimation-Inductive Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES), Zeta potentiometry, X-ray diffraction (XRD), and transmission electron microscopy (TEM). UV peak at 453.5 nm confirmed the formation of AgNPs. XRD studies disclosed the crystalline nature of AgNPs. ICP-AES studies showed the presence of silver in colloidal dispersion and images were recorded by using HRTEM. Synthesized AgNPs were found to be potent against Staphylococcus aureus, Pseudomonasaeruginosa, E. coli, and MRSA. However, these SNPs are cytotoxic in nature and could obey as a superior antibacterial for therapy of bacterial infections in this period of multiple drug defiance.