Abstract
Summary Normal callus cultures of Bidens alba and Bidens pilosa failed to synthesize polyacetylenes (PAs) after four subcultures in spite of modifications to nutritional and environmental parameters. Crown galls, induced on both species by two different strains of Agrobacterium tumefaciens, produced PAs similar to the host tissue but at lower levels. Transformed callus obtained from these galls produced PAs for several years. Changes in composition over time occurred and related PAs not found in the plant were produced by transformed cultures. Neither roots nor light was required for synthesis of PAs. Crown gall callus grown on SH medium with an increased carbohydrate/nitrate ratio showed an average increase of 280% in P A concentration.
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