Synthesis of poly lactic acid (PLA)/nanochitosan–based for bioscaffold materials with the addition of Zn-curcumin

Abstract

The use of chitosan as a bioscaffold material has been carried out many times, but the results obtained are known to be a mixture of the two materials resulting in a less homogeneous bioscaffold which affects to the tensile strength of the bioscaffold. The novelty of this research is to examine the characteristics of the PLA/nanochitosan based bioscaffold material with the addition of Zn-curcumin. The addition of Zn-Curcumin has potential as a composite nanofiber for bone tissue scaffolding through cellular morphology and MTT. The results of the highest tensile strength value were obtained in sample 3 with a composition of 5% nanochitosan concentration. The synthesized PLA has a wavelength value similar to the structure of poly lactic acid characterized by the presence of functional groups –OH, C-H, C = O, C-H(–CH3), and C-O. The morphological characteristics of the sample with a 1.5% Zn-curcumin composition showed an interaction between PLA/nanochitosan with a high homogeneity. The test results show that Zn-Cur containing nanofiber better supports cellular adhesion, dispersion and proliferation compared to other nanofibers. Moreover, it turns out that the addition of Zn-Cur can increase ALP activity and the production of mineralized matrix Zn-CUR complex not only improves osteogenic performance but also has good antibacterial activity.