Abstract
A blue-fluorescence 2,4,6-tris(4-fluorophenyl)-1,3,5-triazine (TFPT) monomer was synthesized with high yield and high purity under mild reaction conditions. The TFPT, which had three active fluoric groups, was facilely incorporated into stable cross-linked fluorescent polymeric nanoparticles (FCPNs) via precipitation polymerization with 6-(4-hydroxyphenyl)pyridazin-3(2H)-one (HPZ). The FCPNs were highly dispersible in water and showed uniform size, intense blue fluorescence, and excellent biocompatibility, making them promising for live cell imaging label applications. This work has the potential to promote the exploitation of novel s-triazine monomers, and to provide a new view of functional crossing-linked polymers based on such monomers.
Highlights
Advances in nanotechnology techniques for diagnostics have created new avenues for bioimaging, drug delivery, bioanalysis, and other biomedical applications, because only a small sample volume is required [1,2,3,4]
We report for the first time the synthesis and characterization of cross-linked s-triazine-containing poly(aryl ether)s nanoparticles, and demonstrate their application as an optical indicator for the fluorescent labeling of E. coli bacteria
A biocompatibility assessment was carried out to evaluate the potential of the s-triazine and HPZ containing fluorescent polymeric nanoparticles (FCPNs) for biomedical applications; this was achieved by measuring the viability of bacteria E. coli after the cells were incubated with different concentrations of FCPNs for 24 h
Summary
Advances in nanotechnology techniques for diagnostics have created new avenues for bioimaging, drug delivery, bioanalysis, and other biomedical applications, because only a small sample volume is required [1,2,3,4]. Fluorescent labeling technologies have been applied widely in bioimaging, biolabeling, biodetection, immunoassays, and other biomedical applications [5,6,7] Traditional fluorescent materials such as organic fluorescent dyes and quantum dots (QDs) have attracted much attention [8,9]. The introduction of these stable and fluorescent triazine units into an FCPN system could be advantageous for stability, as well as the manipulation of the fluorescent state of the polymers In this context, we report for the first time the synthesis and characterization of cross-linked s-triazine-containing poly(aryl ether)s nanoparticles, and demonstrate their application as an optical indicator for the fluorescent labeling of E. coli bacteria (which were used as a model system). We demonstrated the practical application of these nanoparticles for the fluorescent labeling of live E. coli
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