Abstract
Nano peel solution was prepared using Sol-gel technology at a temperature of (80) Celsius. A number of tests were performed to describe the properties and structure of Nano material, including scanning probe microscopy (SPM), which showed the symmetric cumulative distribution of the solution Nanoparticles and the average grain size equal to 64.5 nm and the identical distribution of the Nanoparticles with a diameter of 37.75 nanometers, with a measurement area ranging between (1531.23-1558.19) nanometers. Nano-solution analyzed with a scanning electron microscope (SEM), Inspect type (S50), with a magnification power up to X2000, where dense flakes of Nano particles with a diameter of (50 ± 10) nanometers were observed. The antibacterial activity of Nano-solution by using gram- positive bacteria St. aurous and gram-negative E. coli show the inhibition diameter of (St. aurous) was 27 mm and 25 mm in (E. coli).
Highlights
Study of Nanomaterial is recently widespread due to their large space of Sol–gel used [1]
Experimental Procedure Preparation of Nano-Pomegranate Peel Solution 100 g of pomegranate peel collected washed with tap water exposed the peels to sunlight for two days after derided grinding by a coffee grinder to a fine powder. 1 gram of crusts powder is taken and dissolved with 100 ml quantity of placed in a glass beaker of 1 liter capacity and stirred using Ultrasonic Probe sonicator type (USA) and add to the Beaker 10 ml of concentrated acetic acid with the addition of 100 ml of distilled water and by continuous stirring at a temperature of 80 ° C for 4 hours for 10 days
Antibacterial Activity of Nano Solution The diffusion method was used to evaluate antimicrobial activity by using of Mueller-Hilton agar medium inoculated with bacterial suspension incubated at 35 °C for 24 h., inhibition zones were measured considered as antibacterial activity [9]. 3
Summary
Study of Nanomaterial is recently widespread due to their large space of Sol–gel used [1]. Nanoparticles made in Sol-gel application considered safe theoretically and when conjugated with herbal extract may potentially activate the activity of herb besides producing nano herbal extract nontoxic and valuable in the natural system [2]. Nano herbal extract is distinguished from others by its their small size, high surface-to-volume ratio, electronic properties, functional groups and aggregation behavior, high efficiency, and ability to penetrate the tissues of different organs as a good source of drug for the treatment of many diseases [3, 4]. Pomegranate peel considers as a rich source of polyphenols with potent antioxidant properties [5] and antibacterial properties. The presences study concerns with the preparation of Nanomaterial by Sol-gel methods and characterization
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