Synthesis of Molecularly Imprinted Polymer and Its Application as Solid-Phase Extraction Sorbent for Ceftazidime Determination in Human Serum and Urine Samples

Abstract

A novel molecularly imprinted solid–phase extraction method was developed coupled with HPLC for ceftazidime determination in biological fluids. Various parameters such as molecular recognition properties, adsorption capacity and selectivity of the molecularly imprinted polymer (MIP) were evaluated. The optimal conditions for solid-phase extraction consisted of conditioning with 5 mL of methanol and 20 mL of deionized water at neutral pH, aqueous sample loading (50 mL at pH 3.0), washing using 5 mL of acetone and elution with 5 mL of acetonitrile–water solution (2 : 8, v/v). The MIP selectivity was evaluated by checking several substances with molecular structures similar to that of ceftazidime. The MIP relative standard deviation was 4.1% (n = 5). The limits of detection and quantification for ceftazidime were 7 and 25 μg/L, respectively, while linearity range was 25–800 μg/L.