SYNTHESIS AND APPLICATION OF MOLECULARLY IMPRINTED POLYMERS FOR SOLID-PHASE EXTRACTION OF DIPYRIDAMOLE FROM COMPLEX BIOLOGICAL FLUIDS

Abstract

In this study, a novel sample clean-up technique based on the molecularly imprinted solid-phase extraction (MISPE) procedure is described for the determination of dipyridamole (DIP) in biological fluids. The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linker, and DIP as the template molecule in chloroform. The novel imprinted polymer was used as a solid-phase extraction sorbent for the extraction of DIP from human serum and urine. Various parameters affecting the extraction efficiency of the polymer were evaluated. The optimal conditions for molecularly imprinted solid-phase extraction of the cartridges were obtained. The MIP selectivity was evaluated by checking several substances with similar molecular structures to the template. The limit of detection (LOD) and limit of quantification (LOQ) for DIP in urine samples were 1.6 and 4.0 µg L−1, respectively. These limits for DIP in serum samples were 2.5 and 6.2 µg L−1, respectively. The recoveries for serum and urine samples were higher than 80.0 and 83.0%, respectively.