Synthesis of Leucaena mediated silver nanoparticles: Assessing their photocatalytic degradation of Cr (VI) and in vitro cytotoxicity against DLA cells

Abstract

The development of dependable, environmentally benign methods for the synthesis of nanoscale material is an important concern of nanotechnology. In this work, it explores and finds the synthesis of highly dispersed silver nanoparticles (Ag-NP’s) with the help of Leucaena Leucocephala leaf extract (LLLE) as reducing agent. The synthesized Ag-NP’s was characterized by Fourier Transform Infrared spectroscopy (FT-IR), UV–vis Spectroscopy (UV–vis), X-ray diffraction (XRD) studies, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray spectroscopy (EDX). Moreover, the synthesized Ag-NP’s show the circular shape with average particle size of 40 nm. Also the synthesized Ag-NP’s show better activity towards degradation of aqueous hexavalent chromium solution under visible light irradiation. Brunauer-Emmett-Teller (B.E.T) surface area analysis was done to explain the photodegtadation of chromium in aqueous solution. The short term invitro cytotoxicity of the biosynthesized Ag-NP’s was tested against DLA cell lines using trypan blue dye exclusion technique and thus the synthesized Ag-NP’s show 100% inhibition with concentration of 100 μg/ml.