Abstract
[guanidino-13C]Creatine has been synthesized by reaction involving synthesis of [13C]cyanamide followed by its condensation with sarcosine. The creatine guanidino carbon 13C NMR frequency shifts upon the formation of phosphocreatine by 0.83 ppm with a splitting (2JCP = 3.3 Hz) due to two-bond phosphorus-carbon coupling. The high 13C enrichment (99%) at the guanidino carbon of creatine gave rise to an adequate signal-to-noise ratio for both creatine and phosphocreatine so that a study of the time course of the creatine phosphokinase reaction could be performed and provided the means for a determination of the Km (15.5 mM) of the reaction.
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