Synthesis of DNA, RNA, protein and glycoprotein in mitochondria of cells transformed with Rous sarcoma viruses

Abstract

Summary Mitochondrial DNA and RNA synthesis in Rous sarcoma virus (RSV) transformed chick embryo fibroblast (CEF) cells pretreated with 10 mg per ml of camptothecin was elevated over that in normal CEF cells. Protein synthesis in the RSV-CEF cell mitochondria was also elevated when measured in the presence of 100 μg per ml of cycloheximide. Experiments with temperature sensitive mutants of RSV (TS-68) and RAV-CEF cells indicated the elevation in mitochondrial synthesis was directly related to cell transformation and not merely to cell viral infection. In related experiments, isolated pure mitochondrial preparations from the RSV-CEF cells synthesized greater amounts of glycoprotein autonomously than normal uninfected CEF controls.