Synthesis of cross-linked polymer based on purified Sterculia foetida L. gum as a potential hemostatic agent

Abstract

A method for purifying the polysaccharides from Sterculia foetida L. gum was developed. The effects of purifying conditions were also studied. Results have shown that the conditions of pH 0.5, temperature of 80°C, and 2.5-h reaction duration gave the best polysaccharides yield. The mineral content and the molecular weight of the obtained polysaccharides were measured for characterization. The cross-linking of the polysaccharides with citric acid in order to achieve a desired polymer was performed successfully. The obtained polymers under different conditions were evaluated for the potential application as a hemostatic agent, including tests of swelling behavior in different fluids, whole-blood clotting time and enzymatic bio-degradation. Results have shown that the citric acid/polysaccharides ratio of 0.01 produced a comparative yield of polymer flakes with increased swelling properties, as well as shortened in vitro blood clotting time and gradual biodegradability in three experimental days. Therefore, the citric acid/polysaccharides ratio of 0.01 was selected for the subsequent experiment to obtain the polymer after 1.5 h of purification, which gave the best swelling properties, 20 times the initial weight, reduced the whole-blood clotting time by 50% and showed a rapid bio-degradation. The results obtained from this study provide essential knowledge on the research of the use of S. foetida gum polysaccharides as a passive hemostatic agent, thereby extending the potential pharmaceutical applications of natural polysaccharides in Vietnam.