SYNTHESIS OF BIOACTIVE MEMBRANES FOR GUIDED TISSUE REGENERATION (GTR): A COMPARATIVE STUDY OF THE EFFECT SILANE-BASED CROSS-LINKER

Abstract

Membranes for GTR (Guided Tissue Regeneration) are a promising method for treating periodontitis cases. The manufacture of membranes for GTR based on chitosan, gelatin, and carbonated hydroxyapatite (CHA) by crosslinker tetraethyl orthosilicate (TEOS) and (3-aminopropyl)triethoxysilane (APTES) using the freeze-drying method has been successfully carried out. This research is intended to obtain the optimum synthesis method in the manufacture of membranes with the addition of TEOS and APTES cross-linkers. The membrane was synthesized using a ratio of chitosan (CH) and gelatin (G) = 1:2 with a concentration of TEOS and APTES of 1 mol. Membrane synthesis was carried out using 4 (four) different methods, namely G/CH/CHA, G/CH/CHA+TEOS, G/CH/CHA+APTES, and G/CH/CHA+TEOS-APTES. The addition of silane crosslinking agents was intended to increase the stability of the membrane so that it has high mechanical strength. The synthesized membranes were characterized using SEM-EDX, FTIR, XRD, tensile strength test, and water absorption test. The results of SEMEDX characterization show that the G/CH/CHA+TEOS samples have a higher number of pores, this is supported by higher absorption capacity data compared to the G/CH/CHA+APTES and G/CH/CHA+TEOS-APTES. The tensile strength test shows that the G/CH/CHA+TEOS sample has the highest tensile strength, while XRD data showed insignificant differences in crystallinity between synthesis methods. FTIR spectra all show the typical adsorption bands of gelatin and chitosan the formation of hydrogen bonds and silica networks in the membrane. The synthesis G/CH/CHA+TEOS method is proven to be more effective in obtaining the membrane with the highest tensile strength 2.6 ± 0.15 MPa, and absorption capacity of 451.4 ± 135.24%.