Synthesis of Bioactive Chemicals Cross-linked Sodium Tripolyphosphate (TPP) - Chitosan Nanoparticles for Enhanced Cytotoxic Activity against Human Ovarian Cancer cell Line (PA-1)

Abstract

Currently, nanotechnology makes an impact in all spheres of human life and creates increasing excitement especially in the field of biomedicine and bionanotechnology. The important symbiosis between nanotechnology and green chemistry is the use of bioactive chemicals for the synthesis of nanoparticles. The present research was focused on fabrication of biomolecules loaded chitosan nanoparticles using Gymnema sylvestre leaf extract (GSCNPs). The experiment was performed using ionic gelation technique and was characterized using FTIR, TEM, XRD, Zeta potential and pdi. The morphological investigation showed the presence of smooth spherical surfaced GSCNPs with 58-80 nm in size. The XRD results indicated the crystalline nature of the synthesized nanoparticles. The FTIR results showed the absorption peaks stretching from higher wavelength region to lower region demonstrating the interaction of tripolyphosphate with chitosan and biomolecules. The zeta potential revealed the positive surface charges proving the stability of the synthesized nanoparticles and homogeneous dispersion was presented by the polydispersity index. The cytotoxic activity was performed against PA-1 cell line and the IC50 values obtained were 931 µg/ml for G. sylvestre leaf extract (GSLE) and 378.4 µg/ml for GSCNPs respectively. The present study strongly suggested that the fabricated phytochemicals cross-linked chitosan nanoparticles from Gymnema sylvestre leaf extracts exhibited enhanced cytotoxic activity and it could be effectively used as a promising anticancer agent in the near future.