Synthesis of AuNPs-GH composites for the electrochemical detection of daphnetin

Abstract

Daphnetin plays an important role in biomedicine and is also involved in various physiological functions. It is very important to detect daphnetin accurately and rapidly. A high sensitive voltammetric platform for daphnetin detection was developed based on a gold nanoparticle-doped three-dimensional reduced graphene hydrogel. The synergistic effects between gold nanoparticle (AuNPs) and graphene (GH) drastically enhanced the electrochemical activity of the resulting materials. The morphology and structure of AuNPs-GH composite were characterized using X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM). This modified electrode affords differential pulse voltammetry (DPV) analysis in the linear range of 50 ~ 100 nM daphnetin with a 1.0 nM low detection limit (S/N = 3). The developed method was successfully applied to determine daphnetin in pharmaceuticals with satisfactory recoveries, which shows that the fabricated modified electrode has potential in pharmaceutical analysis.