Synthesis, Characterization, Molecular Docking and Biological studies on Novel Pyazole fused Indole Derivatives

Abstract

The present work deals with the sequence of Novel Pyrazole fused Indole derivatives(4a-4l) were synthesized by simple conventional method andscreened for anthelmintic, antibacterial and anticancer activities. The molecular docking studies were also performed. All of the newly synthesizedcompounds were structurally characterized on the basis of IR, 1HNMR and Mass spectral analysis. Further, all of the newly prepared pyrazole fusedindole derivatives were screened for anthelmintic activity by using Albendazole as standard drug. The antibacterial activity was carried out by agardiffusion (Cup plate) method by using Streptomycin as standard and anticancer activity against MCF-7 cell lines by MTT assay method. The resultsshowed that some of the compounds 4b, 4c and 4f exhibited good anticancer activity. The compounds 4c, 4f, 4j showed good anthelmintic activity and4b,4h and 4i exhibited potential antibacterial activity by comparing with standard drug. Additionally, the molecular docking studies of novel pyrazolefused Indole derivatives was also carried out to explain putative bonding interaction between the active site of EGFR enzyme and synthesized NovelPyrazole fused Indole derivatives by Schrodinger suite.