Synthesis, characterization, biological activity and electrochemistry studies of iron(III) complex with curcumin-oxime ligand.

Abstract

Iron overload is a key target in drug development. This study aimed to investigate the coordination of Fe(III) ions with a curcumin-oxime ligand that may be used in the treatment of iron overload. The synthesis of the curcumin-oxime ligand and curcumin-oxime-Fe(III) complex was successfully made and characterized inits solid-state and solution-state using FT-IR, UV-Vis, elemental analysis, and1 H-NMR. However, in this study, we investigated the apoptotic effects of the curcumin-oxime Fe (III) complex on SW480. SW480 cells were exposed to 99.2% medium for 48hours. After 48hours, the incubation period, cells were harvested by centrifugation and washed in phosphate-buffered saline (PBS) and lysed in radio-immunoprecipitation assay (RIPA) buffer for 20minutes and supernatants were taken and pellets were discarded. ELISA test was used to examine the expression, and activity of cleaved caspase-3, Bax, and Bcl-2 proteins in SW480 cells. ELISA test results indicated that the activities of apoptotic proteins Bax, caspase 3 and Bcl-2 in human SW480 cell lines significantly increased in 48hours treatment. Also, the activity of Bcl-2 was observed to decrease significantly. Catalase activities of the complex were investigated. The findings showed that the complex has a catalase activity. The findings suggest that this type of complex may constitute a new and interesting basis for the future search of new and more potent drugs. The SOD activity of the result showed that the complexes possessed a considerable SOD activity with an IC50 value of 7.685µM. Also, when compared with the control, a complex increased the SOD levels (P<.05). Electrochemistrystudies in the literature have shown that the Fe3+ /Fe2+ couple redox processoccurs in low potential. This value is within the range of compounds that are expected to show superoxide dismutase activity. The Ipc /Ipa shows that one electron transport takes place in the complex. Our results suggest thatcurcumin-oximemay represent a new approach in the treatment of iron overload.